Coding

Part:BBa_K4146220:Design

Designed by: Cai xinyi   Group: iGEM22_BUCT   (2022-09-19)


caffeine synthase gene TcAncCS1, a transmethylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Choose double enzyme digestion, try to make the end of the vector as specific as possible to prevent self-ligation. Note the difference between CS1 and CS2.


Source

CS stands for caffeine synthase, while the TcAncCS1 ancestral gene is derived from Cocobroma, the duplication of TcAncCS1 at node O leads to TcAncCS2.

References